In the present study , an omanental flower sinningia speciosa was transformed with tmek2 and tmek2mut : , the wild type and mutant type of mapkk from tomato , by agrobacterium tumefaciens 本研究用野生型、突变型mapkk基因转化观赏花卉大岩桐，为进一步研究mapkk基因的功能奠定基础，也为花卉植物的转基因提供实验系统。
The segregation ratio of wild type plants to mutant plants in the population degenerated from heterozygotes was 3 : 1 , indicating that the mutant is controlled by a single recessive gene , and designated tentatively as tor 同时，杂合株系呈典型的3 : 1分离比例，表明该突变性状由单隐性基因控制，并将该突变体暂时命名为tor 。
In order to detect the translation profile of ha34 , we expressed this gene in e . coli and generated rabbit polyclonal antibody . no significant signal was detected by hybridizing with hzaml infected by wild type hasnpv 对ha34进行了原核表达并制备了相应的抗体，用该抗体对感染hasnpv的hzam1细胞进行了westernblot检测。
The two researchers and their colleagues took a mixture of “ wild type ” mosquitoes and those that had had sm1 added to their genomes and fed them on mice infected with malarial parasites 这两位研究员和他们的同事一起将一些“野生”的蚊子和那些已经在其基因组中带有sm1基因片断的蚊子混合在一起，用感染疟原虫的老鼠来喂养他们。
After the second pcr amplification , 10 differential cd na fragments were identified among which 6 fragments were wild type specific and 4 fragments were ast mutant specific . all of the 10 differential cdna fragments were sequenced 二次pcr扩增后，进一步筛选出10个差异表达的cdna条带，其中6个是野生型特异表达的， 4个是突变型特异表达的。
Different hetrograft combinations were created between wild type arabidopsis ( c24 ) , mp17 : gfp - transgenic arabidopsis named at9 , and the lew2 - 1 mutant with a phenorype of collapsed xylems , for studying the macromolecule transportation 以野生型拟南芥c24 、转病毒运动蛋白mp17 ： gfp的拟南芥植株at9与lew2 - 1拟南芥突变体建立了不同的异体嫁接组合。
In most wild type e . coli strains containing heterologous pha synthase genes , the amount of pha accumulation was almost undetectable while in e . coli km32b the amount of pha accumulation were clearly visible 在多种野生型大肠杆菌中，尽管使用了各种不同的表达载体，也很难获得可供检测的pha ；但应用大肠杆菌的fqdb缺失突变株却可以获得较多量的pha 。
Methode competence is induced in the s . pn detached from clinic and creat the come duplicaion mutants by insertion - duplication . use the real - time rt - pcr to test the expressing level of vurilence between the mutants and the wild type 8的ctm中生长的菌作为阴性对照。分别在生长的不同时间点提取rna ，最后采用rea卜timert ? pcr来定量测定各种毒力因子mrna表达的水平。
Secondly another vector phz1117 which for replacement of sc ( j11 . 17 / scd78 . 01 , was constructed . phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained 其次，构建了另一个用于置换scq11 . 17 scd78 . 01的置换载体phz1117 ，得到基因置换菌株hxy3并通过southern杂交验证，与野生型m145相比，没有观察到hxy3有明显的表型变化。
After the treatment of different concentrations nacl ( 0 - 200mmol / l ) , the fresh weight of the transgenic lines was dramaticly higher than that of wild type plants , the dry weight had no markedly differences between the transgenic plants and wild plants 在用不同浓度的naci （ 0 200mm 。 l l ）处理后，测其干、鲜重，结果表明转基因植株鲜重明显高于对照，干重差异不显著。
